{"id":9756,"date":"2025-03-31T15:20:18","date_gmt":"2025-03-31T19:20:18","guid":{"rendered":"https:\/\/neuroid.uprrp.edu\/?page_id=9756"},"modified":"2025-03-31T15:56:14","modified_gmt":"2025-03-31T19:56:14","slug":"omaris-y-de-pablo-crespo-2023-2024","status":"publish","type":"page","link":"https:\/\/neuroid.uprrp.edu\/index.php\/omaris-y-de-pablo-crespo-2023-2024\/","title":{"rendered":"Omaris Y. de Pablo Crespo (2023-2024)"},"content":{"rendered":"\t\t
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\n\t\t\t\n\t\t\t\t \n\t\t\t\t\t\tHome Research\t\t\t\t\t<\/span>\n\t\t\t\t\t\n\t\t\t\t\t\t\t<\/h2> <\/span>\n\n\t\t\t\n <\/div>\n\n\t\t\n\t\t<\/div>\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Mentor\/s:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Dr. Alfredo Ghezzi<\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project Title:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Tip60 as a key regulator of PDF neuropeptide release in alcohol-induced neuroadaptations<\/span><\/span>\u00a0<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project Description:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Alcohol consumption is a leading cause of global mortality, responsible for 2.6 million deaths annually and associated with over 200 health conditions. Despite its widespread impact, the epigenetic mechanisms underlying alcohol-induced neurological and behavioral disorders <\/span>remain<\/span> poorly understood. This project investigates the role of Tip60, a histone acetyltransferase critical for chromatin remodeling and transcription, in alcohol-induced disruptions of neuronal function and sleep-wake cycles using a Drosophila model. Tip60 regulates the axonal growth of small ventrolateral neurons (<\/span>sLNvs<\/span>), which produce the pigment-dispersing factor (PDF) neuropeptide essential for circadian rhythms. Alcohol exposure is hypothesized to alter Tip60-mediated transcriptional adaptations, impairing PDF release and <\/span>sLNv<\/span> morphology, leading to disrupted sleep-wake cycles and alcohol tolerance. Using the UAS-GAL4 system, we manipulate Tip60 expression in <\/span>LNv<\/span> and analyze the effects of alcohol exposure on PDF release, neuronal morphology, and sleep behavior. Preliminary findings reveal that Tip60 knockdown disrupts neuronal morphology and PDF release following alcohol exposure, <\/span>identifying<\/span> Tip60 as a key epigenetic regulator of alcohol responses. This research provides insights into the molecular basis of alcohol-induced neuronal and behavioral changes, offering potential targets for therapeutic intervention.<\/span><\/span>\u00a0<\/span>
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\n\t\t\t\n\t\t\t\t \n\t\t\t\t\t\tSummer Research\t\t\t\t\t<\/span>\n\t\t\t\t\t\n\t\t\t\t\t\t\t<\/h2> <\/span>\n\n\t\t\t\n <\/div>\n\n\t\t\n\t\t<\/div>\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Mentor\/s:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Maria Lehtinen<\/span><\/span> , PhD<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project Title:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Choroid plexus immune cell infiltration following neuroinflammation during development<\/span><\/span>\u00a0<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project Description:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Hydrocephalus is a severe condition caused by excessive cerebrospinal fluid (CSF) accumulation in the brain\u2019s ventricles, with pediatric cases often linked to brain inflammation. The choroid plexus (<\/span>ChP<\/span>), a specialized secretory tissue producing most CSF and forming a critical brain-periphery barrier, plays a key role in this process. During brain inflammation, the <\/span>ChP<\/span> interacts with immune cells, but the underlying mechanisms <\/span>remain<\/span> unclear. Existing hydrocephalus mouse models lack representation of inflammatory processes in developing mice. To address this, we <\/span>established<\/span> an inflammatory hydrocephalus model in embryonic and postnatal mice using weight-adjusted doses of lipopolysaccharide (LPS). In embryos (E14\/E15), LPS induced early infiltration of neutrophils and undifferentiated monocytes (CD45+\/S100A9+\/Iba1-), followed by an increase in resident macrophages (CD45+\/Iba1+\/S100A9+) at <\/span>72 hours<\/span>. In contrast, P7 mice showed no significant increase in CD45+ cells with similar dosing. These findings mirror immune responses <\/span>observed<\/span> in adult models and provide insights into the inflammatory mechanisms of hydrocephalus in development. This work advances the potential for targeted therapies aimed at controlling <\/span>ChP<\/span>-driven brain inflammation and mitigating hydrocephalus in pediatric patients.<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Home Research Mentor\/s:\u00a0 Dr. Alfredo Ghezzi Project Title:\u00a0 Tip60 as a key regulator of PDF neuropeptide release in alcohol-induced neuroadaptations\u00a0 Project Description:\u00a0 Alcohol consumption is a leading cause of global mortality, responsible for 2.6 million deaths annually and associated with over 200 health conditions. Despite its widespread impact, the epigenetic mechanisms underlying alcohol-induced neurological and … <\/p>\n

Read more “Omaris Y. de Pablo Crespo (2023-2024)”<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-9756","page","type-page","status-publish","hentry"],"rttpg_featured_image_url":null,"rttpg_author":{"display_name":"NeuroID Program","author_link":"https:\/\/neuroid.uprrp.edu\/index.php\/author\/neuroid\/"},"rttpg_comment":0,"rttpg_category":null,"rttpg_excerpt":"Home Research Mentor\/s:\u00a0 Dr. Alfredo Ghezzi Project Title:\u00a0 Tip60 as a key regulator of PDF neuropeptide release in alcohol-induced neuroadaptations\u00a0 Project Description:\u00a0 Alcohol consumption is a leading cause of global mortality, responsible for 2.6 million deaths annually and associated with over 200 health conditions. Despite its widespread impact, the epigenetic mechanisms underlying alcohol-induced neurological and…","_links":{"self":[{"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/pages\/9756","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/comments?post=9756"}],"version-history":[{"count":8,"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/pages\/9756\/revisions"}],"predecessor-version":[{"id":10279,"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/pages\/9756\/revisions\/10279"}],"wp:attachment":[{"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/media?parent=9756"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}