{"id":9797,"date":"2025-05-01T11:32:57","date_gmt":"2025-05-01T15:32:57","guid":{"rendered":"https:\/\/neuroid.uprrp.edu\/?page_id=9797"},"modified":"2025-11-13T10:53:47","modified_gmt":"2025-11-13T14:53:47","slug":"derek-j-colls-garcia-2024-2025","status":"publish","type":"page","link":"https:\/\/neuroid.uprrp.edu\/index.php\/derek-j-colls-garcia-2024-2025\/","title":{"rendered":"Derek J. Colls Garcia (2024-2025)"},"content":{"rendered":"\t\t
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\n\t\t\t\n\t\t\t\t \n\t\t\t\t\t\tHome Research\t\t\t\t\t<\/span>\n\t\t\t\t\t\n\t\t\t\t\t\t\t<\/h2> <\/span>\n\n\t\t\t\n <\/div>\n\n\t\t\n\t\t<\/div>\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Mentor\/s:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Yancy Ferrer Acosta<\/span><\/span>, <\/span>Ph.D<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project Title:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Expression and characterization of the TREM-Like Transcript-1 protein in the brain<\/span><\/span>\u00a0<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project Description:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Neurological disorders like stroke and Alzheimer\u2019s Disease (AD) are characterized by vascular injury, disrupting the blood-brain barrier (BBB) and brain homeostasis. Our study aims to elucidate a role within the brain for TREM-like Transcript-1 (TLT-1), an abundant platelet immunoreceptor that plays <\/span>an important role<\/span> in immuno- hemostasis. Following a pro-inflammatory insult such as sepsis, TLT-1 in platelets is cleaved, releasing soluble TLT- 1 (sTLT-1) into the bloodstream as an important biomarker of coagulation. In microvascular brain endothelial cells, sTLT-1 was shown to affect their actin cytoskeleton by increasing cell area. In vivo studies from our lab show TLT-1 knockout mice have increased brain permeability.\u00a0<\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Nevertheless, the physiological consequences of these results at the CNS level, need to be further explored. TLT-1\u2019s expression was\u00a0<\/span>identified<\/span>\u00a0in our lab in cultured brain cells, including astrocytes, microglia, and neurons by western blot, suggesting a\u00a0<\/span>possible role<\/span>\u00a0for TLT-1 in the central nervous system (CNS). Our studies\u00a0<\/span>identified<\/span>\u00a0TLT-1 protein expression in rat brain by immunofluorescence, colocalizing with brain cells such as astrocytes. Ongoing experiments are directed towards developing an in vitro BBB model to test the effect of sTLT-1 on BBB permeability using resistance measurements. These studies will examine a physiological function of this immunoreceptor in the CNS and explore its relevance in physiological and pathological states. Research into new molecules involved in neurovascular injury can uncover new avenues for therapeutic intervention, such as strategies for repairing the blood-brain barrier or modulating neuroinflammatory responses, which could have broad implications for treating various neurodegenerative diseases<\/span><\/span>.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\n\t\t\t\n\t\t\t\t \n\t\t\t\t\t\tSummer Research\t\t\t\t\t<\/span>\n\t\t\t\t\t\n\t\t\t\t\t\t\t<\/h2> <\/span>\n\n\t\t\t\n <\/div>\n\n\t\t\n\t\t<\/div>\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Mentor\/s:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Srikant Rangaraju, MD, MS | Rashmi Kumari, PhD<\/span><\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project Title:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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The Role of the Potassium Channel Kv1.3 in Neuroinflammation: Type 2 IFN-induced Glial Activation and LPS-induced In Vivo Responses in Genetic Knockout Models<\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Project Description:<\/span><\/span>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Aim 1. To investigate the importance of Kv1.3 in astrocytes under resting and inflammatory contexts using in vitro models. <\/span><\/p>

We will determine Kv1.3\u2019s protein expression in wild-type (WT) and Kv1.3 knockout (KO) primary mouse \u00a0astrocytes we will perform Western Blot and Immunohistochemistry (IHC). We will also evaluate how \u00a0Interferon-y<\/em> (IFN-y<\/em>), and PAP-1 changes pSTAT1\/3 levels in primary astrocytes. These experiments will \u00a0allow us to determine astrocytic immune responses involving the Kv1.3 channel after genetic and pharmacological modifications.<\/p>

Aim 2. To analyze an existing proteomic dataset of WT vs Kv1.3 KO mice model.<\/span><\/p>

We will analyze proteomic data of astrocytes of a neuroinflammatory LPS model in WT vs Kv1.3 KO mice. This will allow us to determine if the immune response seen in vitro are represented in the in vivo models.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Home Research Mentor\/s:\u00a0 Yancy Ferrer Acosta, Ph.D Project Title:\u00a0 Expression and characterization of the TREM-Like Transcript-1 protein in the brain\u00a0 Project Description:\u00a0 Neurological disorders like stroke and Alzheimer\u2019s Disease (AD) are characterized by vascular injury, disrupting the blood-brain barrier (BBB) and brain homeostasis. Our study aims to elucidate a role within the brain for TREM-like … <\/p>\n

Read more “Derek J. Colls Garcia (2024-2025)”<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-9797","page","type-page","status-publish","hentry"],"rttpg_featured_image_url":null,"rttpg_author":{"display_name":"NeuroID Program","author_link":"https:\/\/neuroid.uprrp.edu\/index.php\/author\/neuroid\/"},"rttpg_comment":0,"rttpg_category":null,"rttpg_excerpt":"Home Research Mentor\/s:\u00a0 Yancy Ferrer Acosta, Ph.D Project Title:\u00a0 Expression and characterization of the TREM-Like Transcript-1 protein in the brain\u00a0 Project Description:\u00a0 Neurological disorders like stroke and Alzheimer\u2019s Disease (AD) are characterized by vascular injury, disrupting the blood-brain barrier (BBB) and brain homeostasis. Our study aims to elucidate a role within the brain for TREM-like…","_links":{"self":[{"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/pages\/9797","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/comments?post=9797"}],"version-history":[{"count":16,"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/pages\/9797\/revisions"}],"predecessor-version":[{"id":11272,"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/pages\/9797\/revisions\/11272"}],"wp:attachment":[{"href":"https:\/\/neuroid.uprrp.edu\/index.php\/wp-json\/wp\/v2\/media?parent=9797"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}